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foki linearized template plasmid dna  (New England Biolabs)


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    New England Biolabs foki linearized template plasmid dna
    Foki Linearized Template Plasmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foki+linearized+template+plasmid+dna/FokI/bio_rxiv__2025__09__12__675721-56-7-56
    Average 95 stars, based on 476 article reviews
    foki linearized template plasmid dna - by Bioz Stars, 2026-10
    95/100 stars

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    Plasmid Preparation:

    Article Title: Mechanism of SARS-CoV-2 Nucleocapsid Protein Phosphorylation-induced Functional Switch
    Article Snippet: .. To make SL1-5 RNA, 100 μg of FokI-linearized template plasmid DNA was used in a 1 mL transcription reaction containing 80 mM HEPES pH 8.0, 30 mM Mg(OAc) 2 , 10 mM dithiothreitol (DTT), 5 mM spermidine, 0.01% Triton X-100, 4 mM each of ATP, UTP, and CTP, 8 mM GTP, 5U of yeast inorganic pyrophosphatase (New England Biolabs or in-house purified), and recombinant, in-house purified P266L T7 RNA polymerase; enzyme purifications were performed as described ( , ). ..

    Purification:

    Article Title: Mechanism of SARS-CoV-2 Nucleocapsid Protein Phosphorylation-induced Functional Switch
    Article Snippet: .. To make SL1-5 RNA, 100 μg of FokI-linearized template plasmid DNA was used in a 1 mL transcription reaction containing 80 mM HEPES pH 8.0, 30 mM Mg(OAc) 2 , 10 mM dithiothreitol (DTT), 5 mM spermidine, 0.01% Triton X-100, 4 mM each of ATP, UTP, and CTP, 8 mM GTP, 5U of yeast inorganic pyrophosphatase (New England Biolabs or in-house purified), and recombinant, in-house purified P266L T7 RNA polymerase; enzyme purifications were performed as described ( , ). ..

    Recombinant:

    Article Title: Mechanism of SARS-CoV-2 Nucleocapsid Protein Phosphorylation-induced Functional Switch
    Article Snippet: .. To make SL1-5 RNA, 100 μg of FokI-linearized template plasmid DNA was used in a 1 mL transcription reaction containing 80 mM HEPES pH 8.0, 30 mM Mg(OAc) 2 , 10 mM dithiothreitol (DTT), 5 mM spermidine, 0.01% Triton X-100, 4 mM each of ATP, UTP, and CTP, 8 mM GTP, 5U of yeast inorganic pyrophosphatase (New England Biolabs or in-house purified), and recombinant, in-house purified P266L T7 RNA polymerase; enzyme purifications were performed as described ( , ). ..



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    foki linearized template plasmid dna  (New England Biolabs)
    95
    New England Biolabs foki linearized template plasmid dna
    Foki Linearized Template Plasmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foki+linearized+template+plasmid+dna/FokI/bio_rxiv__2025__09__12__675721-56-7-56
    Average 95 stars, based on 1 article reviews
    foki linearized template plasmid dna - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

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